Model systems for membranes
The Langmuir-Blodgett technique is an ideal method for producing ordered molecular layers. LB films are deposited using a LB trough that allows the controlled transfer of an amphiphatic molecular layer to a suitable solid substrate. The films produced in this way serve as models for biological membranes and as building blocks for biosensors. However, they are also used as anti-reflection layers or in the production of organic semiconductor elements.
LB-Films: Model for Biological Membranes
ToF-SIMS studies for film formation
A biological model membrane was deposited with a LB process. The membrane consisted of two different lipids and an amphipathic protein which were immobilised on a suited substrate by LB transfer. After this preparation the lipid-protein interactions were characterized more precisely by means of ToF-SIMS spectrometry and imaging. Contrary to expectations, these studies showed an opposite distribution of lipid I (dDPPG) with the protein. Further information on the example shown can be found in the corresponding publication.
|Lipid 1 (dDPPG)|
|Lipid 2 (DPPC)|