Analysis of Langmuir-Blodgett Films

Langmuir-Blodgett films can be used as models for biologic membranes, as building blocks for biosensors but also as antireflective layers or in semiconductor technology. A Langmuir-Blodgett film is made when amphipathic molecules are deposited on a solid support material. Films made in this way can be analysed in vacuum. This example shows a biological model membrane: Two different lipids and one amphipathic protein were immobilised by Langmuir-Blodgett transfer. An analysis with Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) imaging unraveled the distribution of the components in the model system. The analysis showed that the distribution of one of the lipids was the opposite of the protein. That made the previously assumed interaction between these components appear improbable. More information about this example can be obtained from the corresponding publication.

Lipid I (dDPPG)
Lipid II (DPPC)
Protein (SP-B)